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KMID : 0357920060400040255
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Korean Journal of Pathology
2006 Volume.40 No. 4 p.255 ~ p.262
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Effects of Selective Cyclooxygenase-2 Inhibitor NS-398 Pretreatment on the Rat Spinal Cord after Contusion Injury
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Cheong Hyeon-Dae
Sung Joo-Kyung
Kang Ku-Seong
Kim Joung-Ok
Kim Jung-Wan
Sohn Yoon-Kyung
Ham In-Suk
Park Tae-In
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Abstract
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Background: Secondary spinal cord injury (SCI) that follows an initial mechanical insult can exacerbate the overall damage, limit the restorative processes and eventually lead to an in- creased neurological deficit. We hypothesized that selective inhibition of cyclooxygenase-2 (COX-2) may decrease the delayed cell death, and so this will contribute to decreased level of the secondary injury.
Methods: The dorsal surface of the cord at the T9 level was subjected to weight drop impact using a 10 g rod. To block COX-2 activation, a selective COX-2 inhibitor (NS-398) was administered (5 mg/kg, i.p.) 15 min prior to SCI. The COX-1, COX-2, Caspase-3 and PGE2 expressions were measured by real time quantitative RT-PCR and fluorescence immunostaining.
Results: Many activated caspase-3 positive cells were observed at 6 h and they increased until 72 h after SCI. The expression of COX-2 peaked at 6 h after SCI, while the COX-1 expression was unaffected. The principal cells that showed a COX-2 expression were the neurons and microglia. Pretreatment with NS-398 caused a significant decrease in the expression of prostaglandin E2 and activated caspase-3 positive cells after SCI.
Conclusion: These data suggest that COX-2 is one of the main factors related with the pathologic deficits from secondary SCI.
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KEYWORD
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Spinal cord injury, Cyclooxygenase 2 inhibitors, NS-398, Cell death
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